Part:BBa_K3028000:Design

N-terminal part of beta-lactamase fused with dCas9 and double terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1693
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  Illegal BamHI site found at 3972
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We had to choose the strongest double terminator for our construction.

Source

This is a part improvement of BBa_K1689013 (https://parts.igem.org/Part:BBa_K1689013).